Freshwater Ascomycetes - Species Monograph

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Genus	Species	Author
Ayria	nubispora	Raja, Ferrer & Shearer
Class	Order	Family
Sordariomycetes	Inc. sed.	Inc. sed.

MycoBank ID: 512705

Synonymy:

Not applicable.

Description

Habit:

Ascomata scattered on surface or partly immersed in submerged wood.

Ascomata:

Ascomata 250–270 × 158–168 µm, superficial or partially immersed, scattered on wood, globose to subglobose, dark brown, membranous, ostiolate with a hyaline neck.

Peridium:

Peridium 18–20 µm wide, tissue composed of textura angularis in surface view; in medial longitudinal section composed of a dark brown outer layer consisting of isodiametric cells occluded by brown amorphous material and a hyaline inner layer made up of elongated, thin-walled cells.

Beak:

Neck 40-–50 × 30–60 µm, cylindrical, aperiphysate.

Hamathecium:

Paraphyses 90–120 × 7–9 µm, sparse, hyaline, septate, somewhat constricted at the septa, attached at the base, cylindrical, slightly tapering towards the apex.

Ascus:

Asci 212–250 × 34–40 µm (mean = 226 × 36 µm, n = 20), unitunicate, cylindro-clavate with a short pedicel, thin-walled, tapering to a rounded apex, lacking an apical pore or ring, deliquescing in water, with eight overlapping uniseriate to biseriate or irregularly arranged ascospores.

Ascospore:

Ascospores 48–56 × 16–20 µm (mean = 52 × 18 µm, n = 25), hyaline, one-celled, ellipsoidal to fusiform, flattened on one side, finely multiguttulate, with bipolar gelatinous appendages at first hood or cap like, unfurling to a long thread-like structure, and with an oval to circular gelatinous sheath that surrounds the ascospores.

Sheath:

Sheath ca. 10–18 µm wide, staining blue in aqueous nigrosin.

Appendage:

Bipolar gelatinous appendages at first hood or cap like, unfurling to a long thread-like structure.

Culture:

Colonies on PYG (Difco) slow-growing, approximately 20 mm diameter in 30 d, grey in the center, black towards the margins; margins uneven, colonies black in reverse. Hyphae hyaline to dark brown, septate, ca. 2 µm wide. Colonies on CMA (Difco) with balsa effuse, mycelium dark brown, black in reverse, hyphae septate, dark brown, ca. 1–2 µm wide, forming ascomata in culture.

Anamorph:

None observed.

Other info

Type & Location:

UNITED STATES. FLORIDA: Marion County, Ocala National Forest, Fore Lake, 29°16' 15"N,81°55'02"W, water temperature 18C, pH 7, on submerged decorticated woody debris, 9 February 2006, Huzefa A. Raja and J.L. Crane, F99-1 (HOLOTYPE = ILL40594).

Other Specimens:

COSTA RICA. Heredia, La Selva, La Selva stream, 10° 25'0.12"N, 84°1'0.45"W, water temperature, 25C, pH 5, on submerged decorticated woody debris, 10 January 2006, Astrid Ferrer and Marlon Salazar, AF268-1; Heredia, La Selva, Arboleda stream, 10°26'0.75N",84°0'0.62"W, water temperature 25C, pH 7, on submerged decorticated wood, 9 January 2006, Astrid Ferrer and Marlon Salazar, AF268-2; Limon, Barra del Colorado and Tortuguero National Park, Las Palmas Stream, 10°35'0.94N",83°31'0.87W", water temperature 25C, pH 5, on submerged decorticated wood, 18 December 2005, Astrid Ferrer and Marlon Salazar, AF268-3.

Substratum:

Submerged, decorticated wood.

Habitat:

Lentic and loctic.

Distribution:

USA (FL), Costa Rica

Pertinent Literature:

Raja, H.A., Ferrer, A. and Shearer C.A. 2009.

Comments:

Additional Pictures

Ascospore apical caps.
Ascospore with sheath stained in aqueous nigrosin.
Partly dishcarged ascus.

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