Freshwater Ascomycetes - Species Monograph

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Genus	Species	Author
Lindgomyces	cinctospora	Raja, A.N. Mill. & Shearer
Class	Order	Family
Dothideomycetes	Pleosporales	Lindgomycetaceae

Genbank ID: 674766 MycoBank ID: 515192

Synonymy:

Description

Habit:

Immersed to partly immersed on submerged wood.

Ascomata:

Ascomata on wood 285–330 × 374–426 µm, subglobose to conical, black, appearing as raised dome-like structures on the substrate, solitary to gregarious, immersed to partly immersed, ostiolate, papillate, clypeate.

Peridium:

Peridium 45–68 µm wide, in longitudinal section composed of an inner layer of hyaline, elongate, angular, thin-walled cells 5–7 × 3–5 µm, and an outer layer of angular cells occluded with brown amorphous material associated with decomposed wood.

Beak:

Beak 40–70 × 85–90 µm, central, conical to cylindrical, aperiphysate.

Hamathecium:

Pseudoparaphyses numerous, sheet-like, septate, constricted at the septa, 1.5–3 µm wide, anastomosing.

Ascus:

Asci 146–198 × 22–35 µm ( = 167 × 29 µm, n = 15), fissitunicate, clavate, broadly rounded at the apex, tapering to a short stipe, thick-walled, with or without an apical chamber.

Ascospore:

Ascospores 40–58 × 10¬¬–18 µm ( = 50 ×14 µm, n = 50), overlapping biseriate, oblong-fusiform, slightly rounded at the apices, hyaline, 1-septate when young, with the primary septum almost median (0.46–0.57; = 0.50, n = 50), becoming 3-septate and brown with age; with one large and one small guttule in each cell, occasionally filled with numerous small lipid droplets; upper cell broader than the lower cell; surrounded by a gelatinous sheath.

Sheath:

Sheath gelatinous, ca. 2 µm wide along the side of the ascospores and 3–4 µm long at the ascospore apices, staining in aqueous nigrosin; in water, sheath becoming amorphous and spreading to ca. 8–12 µm wide.

Appendage:

None observed.

Culture:

Colonies on PYG immersed with a dense mat of aerial hyphae; immersed hyphae brown black.

Anamorph:

None observed.

Other info

Type & Location:

USA. NORTH CAROLINA: Great Smoky Mountains National Park, Big Creek area, 35°45′054″N, 83°06′530″W, water temp 22 C, pH 5, on submerged wood, 2 Jul 2002, HAR & Nate Hamburger, R56-1 (ILL 40791 HOLOTYPUS; culture CBS).

Other Specimens:

TENNESSEE, Great Smoky Mountains National Park, Forge Creek, 35°33′45″N, 83°50′45″W, water temp 19 C, 6 Sep 2005, HAR & ANM, R56-2; TENNESSEE, Great Smoky Mountains National Park, Fletcher Creek, on road to Cosby camp ground, 35°46′52.8954″N, 83°13′0.264″W, on submerged wood, pH 5–5.5, 28 Jul 2008, HAR, ANM, Alberto M. Stchigel & Misericordia Calduch, R56-3; NORTH CAROLINA, Deep Creek, 35°28′23″N, 83°25′39″W, water temp 21 C, pH 5, 16 Jul 2000, Jinx Campbell, A430-1; NORTH CAROLINA, Cheoah Lake, 35°27′06″N, 83°49′25″W on submerged wood, water temp 28 C, pH 4.5, 19 Jul 2000, Jinx Campbell, A430-3.

Substratum:

Submerged woody debris.

Habitat:

Lentic, lotic.

Distribution:

USA (North Carolina, Tennessee)

Pertinent Literature:

Hirayama et al. 2010.

Comments:

Lindgomyces cinctospora (isolate R56-1) was tested for the production of extracellular enzymes in vitro and was found positive for cellulase, endoglucanase, beta-glucosidase, xylanase, amylase and laccase and also caused soft-rot on balsa wood (Simmonis et al 2008), suggesting it may contribute to decay of wood in fresh water habitats (Hirayama et al. 2010).

Additional Pictures

Ascospore sheath stained in aqueous nigrosin.
Unstained ascospore.
Ascospore at ascus apex stained in aqueous nigrosin.
Ascospore sheath stained in aqueous nigrosin.
Ascospore discharge.
Unstained ascospore sheath.
Endoascus apex.

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