Freshwater Ascomycetes - Species Monograph

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Genus	Species	Author
Aliquandostipite	minuta	Raja & Shearer
Class	Order	Family
Dothideomycetes	Jahnulales	Aliquandostipitaceae

MycoBank ID: 511854

Synonymy:

Description

Habit:

Superficial scattered, solitary, on softened, well -decayed wood, subtended by broad, brown, septate hyphae.

Ascomata:

Ascomata on wood ca. 130-150 x 150--160 µm, hyaline to light brown, globose to subglobose, ostiolate; darkened around the ostiole, papillate, immersed to partially superficial, scattered, with broad, brown subtending hypha ca. 5-15 µm wide

Peridium:

Peridial wall composed of large cells, lumen of the cells ca. 5 x 15 µm wide, textura angularis in surface view

Beak:

Papillate.

Hamathecium:

Of pseudoparaphyses 2 µm wide, septate, hyaline, slightly swollen at the apex.

Ascus:

Asci 74-112 x 36-58 µm (mean = 96 x 44 µm; n = 20), sparse, ovoid to broadly clavate, broadly rounded at the apex, fissitunicate (Figs. 8, 9); endoascus 3-5 µm thick, and extending to 110-120 µm from the opening of the ectoascus, with or without an apical chamber, stalk absent or very short; ascus separating from the ascogenous hyphae at maturity, 8-spored.

Ascospore:

Ascospores 42-54 x 10-14 µm (mean = 47 x 12 µm; n = 40), irregularly arranged, fusiform, multiguttulate, one-septate; upper cell slightly broader than basal cell, hyaline, becoming pale brown; ascospores surrounded by a fusiform mucilaginous sheath ca. 5-18 µm long at the ascospore apices and constricted at the midseptum, with numerous filamentous appendages, unleafing from the ascospore sheath in water and forming a fringe around the mid-septum, refractive acicular crystals present in cells of the ascospores after preservation in glycerin or lactic acid.

Sheath:

Fusiform mucilaginous sheath ca. 5-18 µm long at the ascospore apices and constricted at the midseptum.

Appendage:

Appendages, filamentous, unleafing from the ascospore sheath in water and forming a fringe around the mid-septum, ca. 15-20 µm long, staining in aqueous nigrosin, not visible in glycerin or lactic acid.

Culture:

Colonies on peptone yeast extract glucose (PYG) agar growing slowly, reaching ca. 25 mm in 10 days; aerial hyphae white, immersed hyphae brown, effuse; hyphae wide, thick-walled, septate, deeply constricted at the septa; producing a light pinkish brown pigment that diffuses into the surrounding agar.

Anamorph:

None observed.

Other info

Type & Location:

Other Specimens:

USA, Florida, Monroe County, freshwater swamp at Big Cypress National Preserve, 25° 46' 53"N, 81° 05' 31"W, on submerged decorticated wood, water temperature 20° C, pH 7, February 17, 2006. H.A. Raja and J.L. Crane, F117-1. (holotype ILL 40108).

Substratum:

Submerged or water-soaked wood.

Habitat:

Lentic habitats.

Distribution:

North America: USA, Florida

Pertinent Literature:

Raja, H.A. and C.A. Shearer. 2007. Freshwater ascomycetes: Aliquandostipite minuta (Jahnulales, Dothideomycetes), a new species from Florida. Mycoscience 48:395–398.

Comments:

Additional Pictures

Asci
Ascospore discharge.
Small ascomata of A. minuta next to A. khaoyaiensis.

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